Factors influencing DNA extraction from human skeletal remains: bone characteristic and total demineralization process

High efficiency DNA extraction from bone by total demineralization.

In historical cases, missing persons' identification, mass disasters, and ancient DNA investigations, bone and teeth samples are often the only, and almost always the best, biological material available for DNA typing. This is because of the physical and chemical barrier that the protein:mineral matrix of bone poses to environmental deterioration and biological attack. Most bone extraction prot...

Extraction and Analysis of Ancient DNA from Human Remains of Masjede Kabood Burial Site

Extraction of DNA from Human Remains.

Improvements to analytical methods have made it possible for highly discriminative genotypic information to be gleaned from smaller and smaller amounts of sample material. This fact makes it practical to genotype samples or remains consisting of bone and tooth-samples that likely would not have yielded interpretable genotypic results a short time ago. In parallel, there have been improvements t...

Authenticating DNA Extracted From Ancient Skeletal Remains

The survival of DNA, the most informative biological molecule, for periods of at least several thousand years in bone was demonstrated more than four years ago. However, difficulties with authenticating ancient DNA have made diagenetic studies problematic. It is therefore essential that these problems be overcome before the question of DNA survival can be addressed. Here we describe our work wi...

Bioarchaeology: Scientific Studies of Archaeological Human Skeletal Remains

Bioarchaeology is an interdisciplinary academic specialty, which through the scientific analysis and interpretation of archaeological human skeletal remains, bridges the link between the biological sciences, medicine, anthropology and social sciences. The cornerstone of bioarchaeology is the interaction between culture and human biology. Since the study of people and ancient societies is one of...